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recombinant mouse cd39l2  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse cd39l2
    <t>CD39L2</t> selectively releases the β-phosphate of the ADP generated from a kinase reaction. The released free phosphate is detected using phosphate-detection reagents. The rate of free phosphate production reflects the kinetics of the kinase reaction.
    Recombinant Mouse Cd39l2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+cd39l2/pmc03154929-30-0-15?v=R%26D+Systems
    Average 90 stars, based on 1 article reviews
    recombinant mouse cd39l2 - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction"

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0023172

    CD39L2 selectively releases the β-phosphate of the ADP generated from a kinase reaction. The released free phosphate is detected using phosphate-detection reagents. The rate of free phosphate production reflects the kinetics of the kinase reaction.
    Figure Legend Snippet: CD39L2 selectively releases the β-phosphate of the ADP generated from a kinase reaction. The released free phosphate is detected using phosphate-detection reagents. The rate of free phosphate production reflects the kinetics of the kinase reaction.

    Techniques Used: Generated

    A ) pH profile of CD39L2 activity with ADP or ATP at room temperature. CD39L2 has optimal pH at 5.5 and is selectively active on ADP throughout the pH range. B ) First-order-rate constant of CD39L2 for ADP or ATP at pH 7.5. The specific rate constant of CD39L2 for ADP or ATP in the kinase assay buffer at room temperature was determined to be 41.0 or 0.7 nmol·min −1 mM −1 ·µg −1 (the slopes of the curves), respectively. D ) Relative CD39L2 activity at different salt concentrations.
    Figure Legend Snippet: A ) pH profile of CD39L2 activity with ADP or ATP at room temperature. CD39L2 has optimal pH at 5.5 and is selectively active on ADP throughout the pH range. B ) First-order-rate constant of CD39L2 for ADP or ATP at pH 7.5. The specific rate constant of CD39L2 for ADP or ATP in the kinase assay buffer at room temperature was determined to be 41.0 or 0.7 nmol·min −1 mM −1 ·µg −1 (the slopes of the curves), respectively. D ) Relative CD39L2 activity at different salt concentrations.

    Techniques Used: Activity Assay, Kinase Assay

    Kinetic parameters of CD39L2.
    Figure Legend Snippet: Kinetic parameters of CD39L2.

    Techniques Used:

    A ) Rate constants for CD39L2 in the presence of different concentrations of ATP. The reactions were performed with 0.12 µg of CD39L2 in 50 µL of the kinase assay buffer at room temperature. For clarity, only data at 0, 1.25 and 5 mM of ATP are shown. Slopes (s) of the curves represent the rate constants. Elevated phosphate levels in all reactions due to ATP hydrolysis were regarded as background and were subtracted out. B ) ATP inhibition factor ( i ), the ratio of a rate constant in the presence of ATP to the rate constant in the absence of ATP, is plotted versus ATP concentration.
    Figure Legend Snippet: A ) Rate constants for CD39L2 in the presence of different concentrations of ATP. The reactions were performed with 0.12 µg of CD39L2 in 50 µL of the kinase assay buffer at room temperature. For clarity, only data at 0, 1.25 and 5 mM of ATP are shown. Slopes (s) of the curves represent the rate constants. Elevated phosphate levels in all reactions due to ATP hydrolysis were regarded as background and were subtracted out. B ) ATP inhibition factor ( i ), the ratio of a rate constant in the presence of ATP to the rate constant in the absence of ATP, is plotted versus ATP concentration.

    Techniques Used: Kinase Assay, Inhibition, Concentration Assay

    A ) A Glucose curve. All reactions were initiated in the presence of 0.12 mM ATP, 0.2 µg GCK and 0.3 µg CD39L2 in 150 µL assay buffer at room temperature and proceeded for 15 minutes. The reaction that contained no glucose was set as a blank and the OD readings were plotted versus glucose concentration. The curve fit the Michaelis-Menten equation well with K m of 7.23±1.44 mM. B ) An ATP curve. Reactions were performed in the presence of 20 mM glucose, 1 µg GCK and 0.1 µg CD39L2 and variable concentrations of ATP in 50 µL assay buffer at room temperature and proceeded for 25 minutes. The reaction that contained no ATP was set as a blank. For each ATP concentration, a no-kinase negative control was performed for background correction. The OD readings of the reactions (purple) were first corrected with backgrounds subtraction (blue) and further corrected using ATP inhibition factors (red) and finally fit with the Michaelis-Menten equation to obtain a K m around 3.55 mM. C ) A GCK dose curve was performed with 10 mM ATP, 20 mM of glucose and 0.2 µg CD39L2 in 50 µL assay buffer at room temperature. All reactions were proceeded for 20 minutes and the OD was plotted versus GCK input. The reaction that contained no kinase was set as a blank. The slope of the curve, 6.84 OD/µg, corresponded to a specific activity of 2416 pmol/min/µg using Eq.10 ( k 2 = 8.2 nmol·min −1 ·mM −1 ; Vol = 50 µL; t = 25 min; i = 0.271; r = 0.396).
    Figure Legend Snippet: A ) A Glucose curve. All reactions were initiated in the presence of 0.12 mM ATP, 0.2 µg GCK and 0.3 µg CD39L2 in 150 µL assay buffer at room temperature and proceeded for 15 minutes. The reaction that contained no glucose was set as a blank and the OD readings were plotted versus glucose concentration. The curve fit the Michaelis-Menten equation well with K m of 7.23±1.44 mM. B ) An ATP curve. Reactions were performed in the presence of 20 mM glucose, 1 µg GCK and 0.1 µg CD39L2 and variable concentrations of ATP in 50 µL assay buffer at room temperature and proceeded for 25 minutes. The reaction that contained no ATP was set as a blank. For each ATP concentration, a no-kinase negative control was performed for background correction. The OD readings of the reactions (purple) were first corrected with backgrounds subtraction (blue) and further corrected using ATP inhibition factors (red) and finally fit with the Michaelis-Menten equation to obtain a K m around 3.55 mM. C ) A GCK dose curve was performed with 10 mM ATP, 20 mM of glucose and 0.2 µg CD39L2 in 50 µL assay buffer at room temperature. All reactions were proceeded for 20 minutes and the OD was plotted versus GCK input. The reaction that contained no kinase was set as a blank. The slope of the curve, 6.84 OD/µg, corresponded to a specific activity of 2416 pmol/min/µg using Eq.10 ( k 2 = 8.2 nmol·min −1 ·mM −1 ; Vol = 50 µL; t = 25 min; i = 0.271; r = 0.396).

    Techniques Used: Concentration Assay, Negative Control, Inhibition, Activity Assay

    A ) An APS curve. All reactions were initiated with 0.12 mM ATP, 0.1 µg of APSK and 0.3 µg CD39L2 in 150 µL assay buffer at room temperature and proceeded for 10 minutes. The OD was plotted versus the APS concentration and the apparent K m (designated as K m ′) was visually estimated to be about 10 µM. B ) An ATP curve. All reactions were performed in the presence of 100 µM APS, 0.2 µg of APSK and 0.3 µg of CD39L2 in 150 µL assay buffer at room temperature and proceeded for 15 minutes. The obtained OD readings (purple) were first corrected by background subtraction (blue) and then corrected by the ATP inhibition factors (red) and finally fit with the Michaelis-Menten equation to obtain a K m of 126 µM. C ) An APSK enzyme curve. All reactions were performed with 1 mM ATP, 0.1 mM APS and 0.14 µg CD39L2 in 50 µL assay buffer at room temperature for 20 minutes. The OD was plotted versus APSK input. The slope of curve was converted to a specific activity, 1125 pmol/min/µg, using Eq.10 and r = 0.61.
    Figure Legend Snippet: A ) An APS curve. All reactions were initiated with 0.12 mM ATP, 0.1 µg of APSK and 0.3 µg CD39L2 in 150 µL assay buffer at room temperature and proceeded for 10 minutes. The OD was plotted versus the APS concentration and the apparent K m (designated as K m ′) was visually estimated to be about 10 µM. B ) An ATP curve. All reactions were performed in the presence of 100 µM APS, 0.2 µg of APSK and 0.3 µg of CD39L2 in 150 µL assay buffer at room temperature and proceeded for 15 minutes. The obtained OD readings (purple) were first corrected by background subtraction (blue) and then corrected by the ATP inhibition factors (red) and finally fit with the Michaelis-Menten equation to obtain a K m of 126 µM. C ) An APSK enzyme curve. All reactions were performed with 1 mM ATP, 0.1 mM APS and 0.14 µg CD39L2 in 50 µL assay buffer at room temperature for 20 minutes. The OD was plotted versus APSK input. The slope of curve was converted to a specific activity, 1125 pmol/min/µg, using Eq.10 and r = 0.61.

    Techniques Used: Concentration Assay, Inhibition, Activity Assay

    All reactions were initiated with 0.2 mM of ATP, 0.2 mM of myelin basic protein peptide and 0.2 µg of CD39L2 in 50 µL assay buffer and proceeded for 15 minutes. The OD was plotted versus ERK1 input. A reaction containing all components except kinase served as a blank. The slope of the curve (1.215 OD/µg) was converted to a specific activity, 482 pmol/min/µg, using Eq.10 and r = 0.597.
    Figure Legend Snippet: All reactions were initiated with 0.2 mM of ATP, 0.2 mM of myelin basic protein peptide and 0.2 µg of CD39L2 in 50 µL assay buffer and proceeded for 15 minutes. The OD was plotted versus ERK1 input. A reaction containing all components except kinase served as a blank. The slope of the curve (1.215 OD/µg) was converted to a specific activity, 482 pmol/min/µg, using Eq.10 and r = 0.597.

    Techniques Used: Activity Assay

    Blue triangles represent the background. Background reactions contained all components except the kinases. A ) Trials for GCK. Reactions were performed with 20 mM Glucose, 0.1 mM ATP, 0.64 µg GCK and 0.1 µg CD39L2 in 150 µL kinase assay buffer at room temperature for 20 minutes. B ) Trials for APSK. Reactions were performed with 0.0625 mM APS, 0.125 mM ATP, 1 µg APSK, 0.15 µg CD39L2 in 150 µL assay buffer at room temperature for 15 minutes. C ) Trials for ERK1. Reactions were performed with 0.05 mM MBP, 0.1 mM ATP, 0.2 µg ERK1, 0.1 µg CD39L2 in 150 µL assay buffer at room temperature for 20 minutes.
    Figure Legend Snippet: Blue triangles represent the background. Background reactions contained all components except the kinases. A ) Trials for GCK. Reactions were performed with 20 mM Glucose, 0.1 mM ATP, 0.64 µg GCK and 0.1 µg CD39L2 in 150 µL kinase assay buffer at room temperature for 20 minutes. B ) Trials for APSK. Reactions were performed with 0.0625 mM APS, 0.125 mM ATP, 1 µg APSK, 0.15 µg CD39L2 in 150 µL assay buffer at room temperature for 15 minutes. C ) Trials for ERK1. Reactions were performed with 0.05 mM MBP, 0.1 mM ATP, 0.2 µg ERK1, 0.1 µg CD39L2 in 150 µL assay buffer at room temperature for 20 minutes.

    Techniques Used: Kinase Assay



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    recombinant mouse cd39l2  (R&D Systems)
    90
    R&D Systems recombinant mouse cd39l2
    <t>CD39L2</t> selectively releases the β-phosphate of the ADP generated from a kinase reaction. The released free phosphate is detected using phosphate-detection reagents. The rate of free phosphate production reflects the kinetics of the kinase reaction.
    Recombinant Mouse Cd39l2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+cd39l2/pmc03154929-30-0-15?v=R%26D+Systems
    Average 90 stars, based on 1 article reviews
    recombinant mouse cd39l2 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    CD39L2 selectively releases the β-phosphate of the ADP generated from a kinase reaction. The released free phosphate is detected using phosphate-detection reagents. The rate of free phosphate production reflects the kinetics of the kinase reaction.

    Journal: PLoS ONE

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    doi: 10.1371/journal.pone.0023172

    Figure Lengend Snippet: CD39L2 selectively releases the β-phosphate of the ADP generated from a kinase reaction. The released free phosphate is detected using phosphate-detection reagents. The rate of free phosphate production reflects the kinetics of the kinase reaction.

    Article Snippet: Recombinant mouse CD39L2 (ENTPD6), human ERK1, and the Malachite Green Phosphate Detection Kit were from R&D Systems (Minneapolis, MN).

    Techniques: Generated

    A ) pH profile of CD39L2 activity with ADP or ATP at room temperature. CD39L2 has optimal pH at 5.5 and is selectively active on ADP throughout the pH range. B ) First-order-rate constant of CD39L2 for ADP or ATP at pH 7.5. The specific rate constant of CD39L2 for ADP or ATP in the kinase assay buffer at room temperature was determined to be 41.0 or 0.7 nmol·min −1 mM −1 ·µg −1 (the slopes of the curves), respectively. D ) Relative CD39L2 activity at different salt concentrations.

    Journal: PLoS ONE

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    doi: 10.1371/journal.pone.0023172

    Figure Lengend Snippet: A ) pH profile of CD39L2 activity with ADP or ATP at room temperature. CD39L2 has optimal pH at 5.5 and is selectively active on ADP throughout the pH range. B ) First-order-rate constant of CD39L2 for ADP or ATP at pH 7.5. The specific rate constant of CD39L2 for ADP or ATP in the kinase assay buffer at room temperature was determined to be 41.0 or 0.7 nmol·min −1 mM −1 ·µg −1 (the slopes of the curves), respectively. D ) Relative CD39L2 activity at different salt concentrations.

    Article Snippet: Recombinant mouse CD39L2 (ENTPD6), human ERK1, and the Malachite Green Phosphate Detection Kit were from R&D Systems (Minneapolis, MN).

    Techniques: Activity Assay, Kinase Assay

    Kinetic parameters of CD39L2.

    Journal: PLoS ONE

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    doi: 10.1371/journal.pone.0023172

    Figure Lengend Snippet: Kinetic parameters of CD39L2.

    Article Snippet: Recombinant mouse CD39L2 (ENTPD6), human ERK1, and the Malachite Green Phosphate Detection Kit were from R&D Systems (Minneapolis, MN).

    Techniques:

    A ) Rate constants for CD39L2 in the presence of different concentrations of ATP. The reactions were performed with 0.12 µg of CD39L2 in 50 µL of the kinase assay buffer at room temperature. For clarity, only data at 0, 1.25 and 5 mM of ATP are shown. Slopes (s) of the curves represent the rate constants. Elevated phosphate levels in all reactions due to ATP hydrolysis were regarded as background and were subtracted out. B ) ATP inhibition factor ( i ), the ratio of a rate constant in the presence of ATP to the rate constant in the absence of ATP, is plotted versus ATP concentration.

    Journal: PLoS ONE

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    doi: 10.1371/journal.pone.0023172

    Figure Lengend Snippet: A ) Rate constants for CD39L2 in the presence of different concentrations of ATP. The reactions were performed with 0.12 µg of CD39L2 in 50 µL of the kinase assay buffer at room temperature. For clarity, only data at 0, 1.25 and 5 mM of ATP are shown. Slopes (s) of the curves represent the rate constants. Elevated phosphate levels in all reactions due to ATP hydrolysis were regarded as background and were subtracted out. B ) ATP inhibition factor ( i ), the ratio of a rate constant in the presence of ATP to the rate constant in the absence of ATP, is plotted versus ATP concentration.

    Article Snippet: Recombinant mouse CD39L2 (ENTPD6), human ERK1, and the Malachite Green Phosphate Detection Kit were from R&D Systems (Minneapolis, MN).

    Techniques: Kinase Assay, Inhibition, Concentration Assay

    A ) A Glucose curve. All reactions were initiated in the presence of 0.12 mM ATP, 0.2 µg GCK and 0.3 µg CD39L2 in 150 µL assay buffer at room temperature and proceeded for 15 minutes. The reaction that contained no glucose was set as a blank and the OD readings were plotted versus glucose concentration. The curve fit the Michaelis-Menten equation well with K m of 7.23±1.44 mM. B ) An ATP curve. Reactions were performed in the presence of 20 mM glucose, 1 µg GCK and 0.1 µg CD39L2 and variable concentrations of ATP in 50 µL assay buffer at room temperature and proceeded for 25 minutes. The reaction that contained no ATP was set as a blank. For each ATP concentration, a no-kinase negative control was performed for background correction. The OD readings of the reactions (purple) were first corrected with backgrounds subtraction (blue) and further corrected using ATP inhibition factors (red) and finally fit with the Michaelis-Menten equation to obtain a K m around 3.55 mM. C ) A GCK dose curve was performed with 10 mM ATP, 20 mM of glucose and 0.2 µg CD39L2 in 50 µL assay buffer at room temperature. All reactions were proceeded for 20 minutes and the OD was plotted versus GCK input. The reaction that contained no kinase was set as a blank. The slope of the curve, 6.84 OD/µg, corresponded to a specific activity of 2416 pmol/min/µg using Eq.10 ( k 2 = 8.2 nmol·min −1 ·mM −1 ; Vol = 50 µL; t = 25 min; i = 0.271; r = 0.396).

    Journal: PLoS ONE

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    doi: 10.1371/journal.pone.0023172

    Figure Lengend Snippet: A ) A Glucose curve. All reactions were initiated in the presence of 0.12 mM ATP, 0.2 µg GCK and 0.3 µg CD39L2 in 150 µL assay buffer at room temperature and proceeded for 15 minutes. The reaction that contained no glucose was set as a blank and the OD readings were plotted versus glucose concentration. The curve fit the Michaelis-Menten equation well with K m of 7.23±1.44 mM. B ) An ATP curve. Reactions were performed in the presence of 20 mM glucose, 1 µg GCK and 0.1 µg CD39L2 and variable concentrations of ATP in 50 µL assay buffer at room temperature and proceeded for 25 minutes. The reaction that contained no ATP was set as a blank. For each ATP concentration, a no-kinase negative control was performed for background correction. The OD readings of the reactions (purple) were first corrected with backgrounds subtraction (blue) and further corrected using ATP inhibition factors (red) and finally fit with the Michaelis-Menten equation to obtain a K m around 3.55 mM. C ) A GCK dose curve was performed with 10 mM ATP, 20 mM of glucose and 0.2 µg CD39L2 in 50 µL assay buffer at room temperature. All reactions were proceeded for 20 minutes and the OD was plotted versus GCK input. The reaction that contained no kinase was set as a blank. The slope of the curve, 6.84 OD/µg, corresponded to a specific activity of 2416 pmol/min/µg using Eq.10 ( k 2 = 8.2 nmol·min −1 ·mM −1 ; Vol = 50 µL; t = 25 min; i = 0.271; r = 0.396).

    Article Snippet: Recombinant mouse CD39L2 (ENTPD6), human ERK1, and the Malachite Green Phosphate Detection Kit were from R&D Systems (Minneapolis, MN).

    Techniques: Concentration Assay, Negative Control, Inhibition, Activity Assay

    A ) An APS curve. All reactions were initiated with 0.12 mM ATP, 0.1 µg of APSK and 0.3 µg CD39L2 in 150 µL assay buffer at room temperature and proceeded for 10 minutes. The OD was plotted versus the APS concentration and the apparent K m (designated as K m ′) was visually estimated to be about 10 µM. B ) An ATP curve. All reactions were performed in the presence of 100 µM APS, 0.2 µg of APSK and 0.3 µg of CD39L2 in 150 µL assay buffer at room temperature and proceeded for 15 minutes. The obtained OD readings (purple) were first corrected by background subtraction (blue) and then corrected by the ATP inhibition factors (red) and finally fit with the Michaelis-Menten equation to obtain a K m of 126 µM. C ) An APSK enzyme curve. All reactions were performed with 1 mM ATP, 0.1 mM APS and 0.14 µg CD39L2 in 50 µL assay buffer at room temperature for 20 minutes. The OD was plotted versus APSK input. The slope of curve was converted to a specific activity, 1125 pmol/min/µg, using Eq.10 and r = 0.61.

    Journal: PLoS ONE

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    doi: 10.1371/journal.pone.0023172

    Figure Lengend Snippet: A ) An APS curve. All reactions were initiated with 0.12 mM ATP, 0.1 µg of APSK and 0.3 µg CD39L2 in 150 µL assay buffer at room temperature and proceeded for 10 minutes. The OD was plotted versus the APS concentration and the apparent K m (designated as K m ′) was visually estimated to be about 10 µM. B ) An ATP curve. All reactions were performed in the presence of 100 µM APS, 0.2 µg of APSK and 0.3 µg of CD39L2 in 150 µL assay buffer at room temperature and proceeded for 15 minutes. The obtained OD readings (purple) were first corrected by background subtraction (blue) and then corrected by the ATP inhibition factors (red) and finally fit with the Michaelis-Menten equation to obtain a K m of 126 µM. C ) An APSK enzyme curve. All reactions were performed with 1 mM ATP, 0.1 mM APS and 0.14 µg CD39L2 in 50 µL assay buffer at room temperature for 20 minutes. The OD was plotted versus APSK input. The slope of curve was converted to a specific activity, 1125 pmol/min/µg, using Eq.10 and r = 0.61.

    Article Snippet: Recombinant mouse CD39L2 (ENTPD6), human ERK1, and the Malachite Green Phosphate Detection Kit were from R&D Systems (Minneapolis, MN).

    Techniques: Concentration Assay, Inhibition, Activity Assay

    All reactions were initiated with 0.2 mM of ATP, 0.2 mM of myelin basic protein peptide and 0.2 µg of CD39L2 in 50 µL assay buffer and proceeded for 15 minutes. The OD was plotted versus ERK1 input. A reaction containing all components except kinase served as a blank. The slope of the curve (1.215 OD/µg) was converted to a specific activity, 482 pmol/min/µg, using Eq.10 and r = 0.597.

    Journal: PLoS ONE

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    doi: 10.1371/journal.pone.0023172

    Figure Lengend Snippet: All reactions were initiated with 0.2 mM of ATP, 0.2 mM of myelin basic protein peptide and 0.2 µg of CD39L2 in 50 µL assay buffer and proceeded for 15 minutes. The OD was plotted versus ERK1 input. A reaction containing all components except kinase served as a blank. The slope of the curve (1.215 OD/µg) was converted to a specific activity, 482 pmol/min/µg, using Eq.10 and r = 0.597.

    Article Snippet: Recombinant mouse CD39L2 (ENTPD6), human ERK1, and the Malachite Green Phosphate Detection Kit were from R&D Systems (Minneapolis, MN).

    Techniques: Activity Assay

    Blue triangles represent the background. Background reactions contained all components except the kinases. A ) Trials for GCK. Reactions were performed with 20 mM Glucose, 0.1 mM ATP, 0.64 µg GCK and 0.1 µg CD39L2 in 150 µL kinase assay buffer at room temperature for 20 minutes. B ) Trials for APSK. Reactions were performed with 0.0625 mM APS, 0.125 mM ATP, 1 µg APSK, 0.15 µg CD39L2 in 150 µL assay buffer at room temperature for 15 minutes. C ) Trials for ERK1. Reactions were performed with 0.05 mM MBP, 0.1 mM ATP, 0.2 µg ERK1, 0.1 µg CD39L2 in 150 µL assay buffer at room temperature for 20 minutes.

    Journal: PLoS ONE

    Article Title: Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction

    doi: 10.1371/journal.pone.0023172

    Figure Lengend Snippet: Blue triangles represent the background. Background reactions contained all components except the kinases. A ) Trials for GCK. Reactions were performed with 20 mM Glucose, 0.1 mM ATP, 0.64 µg GCK and 0.1 µg CD39L2 in 150 µL kinase assay buffer at room temperature for 20 minutes. B ) Trials for APSK. Reactions were performed with 0.0625 mM APS, 0.125 mM ATP, 1 µg APSK, 0.15 µg CD39L2 in 150 µL assay buffer at room temperature for 15 minutes. C ) Trials for ERK1. Reactions were performed with 0.05 mM MBP, 0.1 mM ATP, 0.2 µg ERK1, 0.1 µg CD39L2 in 150 µL assay buffer at room temperature for 20 minutes.

    Article Snippet: Recombinant mouse CD39L2 (ENTPD6), human ERK1, and the Malachite Green Phosphate Detection Kit were from R&D Systems (Minneapolis, MN).

    Techniques: Kinase Assay